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pegfpc1 fusion mammalian protein expression vector  (TaKaRa)


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    Structured Review

    TaKaRa pegfpc1 fusion mammalian protein expression vector
    Figure 2: Co-immunoprecipitation experiments in HEK293 cells transfected with (A) pEF-BOS-SNAP25-FLAG alone or together with <t>pEGFPC1-TrkB.</t> (B) pEF-BOS-VAMP2-FLAG alone or together with pEGFPC1-TrkB. (C) pEF-BOS-SNAP25-FLAG, pEF-BOS- VAMP2-FLAG and TrkA-HA. (D) pEF-BOS-SNAP25-FLAG, pEF-BOS-VAMP2-FLAG and TrkC-myc. No co-immunoprecipitation was observed between proteins analyzed with anti α-GFP, anti-myc, anti-FLAG, or anti-HA antibodies. Two transfections in HEK cells per condition were run in parallel for each experiment and three experiments were done. Arrows indicate specific bands.
    Pegfpc1 Fusion Mammalian Protein Expression Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 15959 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/expression+vector+pegfpc1/EcoR+I/pm30542508-242-18-24
    Average 99 stars, based on 15959 article reviews
    pegfpc1 fusion mammalian protein expression vector - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "Syntaxin-1/TI-VAMP SNAREs interact with Trk receptors and are required for neurotrophin-dependent outgrowth."

    Article Title: Syntaxin-1/TI-VAMP SNAREs interact with Trk receptors and are required for neurotrophin-dependent outgrowth.

    Journal: Oncotarget

    doi: 10.18632/oncotarget.26307

    Figure 2: Co-immunoprecipitation experiments in HEK293 cells transfected with (A) pEF-BOS-SNAP25-FLAG alone or together with pEGFPC1-TrkB. (B) pEF-BOS-VAMP2-FLAG alone or together with pEGFPC1-TrkB. (C) pEF-BOS-SNAP25-FLAG, pEF-BOS- VAMP2-FLAG and TrkA-HA. (D) pEF-BOS-SNAP25-FLAG, pEF-BOS-VAMP2-FLAG and TrkC-myc. No co-immunoprecipitation was observed between proteins analyzed with anti α-GFP, anti-myc, anti-FLAG, or anti-HA antibodies. Two transfections in HEK cells per condition were run in parallel for each experiment and three experiments were done. Arrows indicate specific bands.
    Figure Legend Snippet: Figure 2: Co-immunoprecipitation experiments in HEK293 cells transfected with (A) pEF-BOS-SNAP25-FLAG alone or together with pEGFPC1-TrkB. (B) pEF-BOS-VAMP2-FLAG alone or together with pEGFPC1-TrkB. (C) pEF-BOS-SNAP25-FLAG, pEF-BOS- VAMP2-FLAG and TrkA-HA. (D) pEF-BOS-SNAP25-FLAG, pEF-BOS-VAMP2-FLAG and TrkC-myc. No co-immunoprecipitation was observed between proteins analyzed with anti α-GFP, anti-myc, anti-FLAG, or anti-HA antibodies. Two transfections in HEK cells per condition were run in parallel for each experiment and three experiments were done. Arrows indicate specific bands.

    Techniques Used: Immunoprecipitation, Transfection

    Related Articles

    Expressing:

    Article Title: Erk1/2 MAPK and Caldesmon Differentially Regulate Podosome Dynamics in A7r5 Vascular Smooth Muscle Cells
    Article Snippet: .. The resulting insert was subcloned into BspEI (after making it blunt with T4 DNA polymerase) and BamHI sites of MCS in mammalian expression vector pEGFPC1 (Clontech). ..

    Article Title: The Truncated C-terminal RNA Recognition Motif of TDP-43 Protein Plays a Key Role in Forming Proteinaceous Aggregates
    Article Snippet: Blots were then incubated with anti-rabbit antibody (Amersham Biosciences) for 1 h, washed three times with TBST, developed by the ECL luminescence kit t8 (Amersham Biosciences), and visualized using the luminescence image analyzer Fuji LAS-1000plus (Fujifilm). .. The full-length cDNA were cloned into XhoI/XbaI sites of expression vector pEGFPC1 (Clontech) using the following primers: 5′-AGAT CTCGAG CGTCTGAATATATTCGGGTAACC-3′ and 5′-TTA TCTAGA CTACATTCCCCAGCCAGAA-3′ (XhoI and XbaI restriction sites are underlined ). ..

    Article Title: Examination of mitochondrial protein targeting of haem synthetic enzymes: in vivo identification of three functional haem-responsive motifs in 5-aminolaevulinate synthase
    Article Snippet: The first contained residues 7–68 of human FC (as annotated in Swiss-Prot entry P22830) fused to the N-terminus of GFP in the expression vector pEGFPN1 (ClonTech). .. The second contained residues 390–423 of FC fused to the C-terminal end of the GFP in the expression vector pEGFPC1 (ClonTech). ..

    Article Title: Plakoglobin but not desmoplakin regulates keratinocyte cohesion via modulation of p38MAPK signaling.
    Article Snippet: .. The yielded amplicons were subcloned into pGEM-T (Promega, Madison, WI) and the respective complementary DNAs subsequently transferred as XhoI- and EcoRI-flanking fragments into the same sites within expression vector pEGFPC1 (Clontech, Mountain View, CA). ..

    Article Title: Examination of mitochondrial protein targeting of haem synthetic enzymes: in vivo identification of three functional haem-responsive motifs in 5-aminolaevulinate synthase
    Article Snippet: The first contained residues 7–68 of human FC (as annotated in Swiss-Prot entry {"type":"entrez-protein","attrs":{"text":"P22830","term_id":"85701348"}} P22830 ) fused to the N-terminus of GFP in the expression vector pEGFPN1 (ClonTech). .. The second contained residues 390–423 of FC fused to the C-terminal end of the GFP in the expression vector pEGFPC1 (ClonTech). ..

    Plasmid Preparation:

    Article Title: Erk1/2 MAPK and Caldesmon Differentially Regulate Podosome Dynamics in A7r5 Vascular Smooth Muscle Cells
    Article Snippet: .. The resulting insert was subcloned into BspEI (after making it blunt with T4 DNA polymerase) and BamHI sites of MCS in mammalian expression vector pEGFPC1 (Clontech). ..

    Article Title: The Truncated C-terminal RNA Recognition Motif of TDP-43 Protein Plays a Key Role in Forming Proteinaceous Aggregates
    Article Snippet: Blots were then incubated with anti-rabbit antibody (Amersham Biosciences) for 1 h, washed three times with TBST, developed by the ECL luminescence kit t8 (Amersham Biosciences), and visualized using the luminescence image analyzer Fuji LAS-1000plus (Fujifilm). .. The full-length cDNA were cloned into XhoI/XbaI sites of expression vector pEGFPC1 (Clontech) using the following primers: 5′-AGAT CTCGAG CGTCTGAATATATTCGGGTAACC-3′ and 5′-TTA TCTAGA CTACATTCCCCAGCCAGAA-3′ (XhoI and XbaI restriction sites are underlined ). ..

    Article Title: Examination of mitochondrial protein targeting of haem synthetic enzymes: in vivo identification of three functional haem-responsive motifs in 5-aminolaevulinate synthase
    Article Snippet: The first contained residues 7–68 of human FC (as annotated in Swiss-Prot entry P22830) fused to the N-terminus of GFP in the expression vector pEGFPN1 (ClonTech). .. The second contained residues 390–423 of FC fused to the C-terminal end of the GFP in the expression vector pEGFPC1 (ClonTech). ..

    Article Title: Examination of mitochondrial protein targeting of haem synthetic enzymes: in vivo identification of three functional haem-responsive motifs in 5-aminolaevulinate synthase
    Article Snippet: The first contained residues 7–68 of human FC (as annotated in Swiss-Prot entry {"type":"entrez-protein","attrs":{"text":"P22830","term_id":"85701348"}} P22830 ) fused to the N-terminus of GFP in the expression vector pEGFPN1 (ClonTech). .. The second contained residues 390–423 of FC fused to the C-terminal end of the GFP in the expression vector pEGFPC1 (ClonTech). ..

    Clone Assay:

    Article Title: The Truncated C-terminal RNA Recognition Motif of TDP-43 Protein Plays a Key Role in Forming Proteinaceous Aggregates
    Article Snippet: Blots were then incubated with anti-rabbit antibody (Amersham Biosciences) for 1 h, washed three times with TBST, developed by the ECL luminescence kit t8 (Amersham Biosciences), and visualized using the luminescence image analyzer Fuji LAS-1000plus (Fujifilm). .. The full-length cDNA were cloned into XhoI/XbaI sites of expression vector pEGFPC1 (Clontech) using the following primers: 5′-AGAT CTCGAG CGTCTGAATATATTCGGGTAACC-3′ and 5′-TTA TCTAGA CTACATTCCCCAGCCAGAA-3′ (XhoI and XbaI restriction sites are underlined ). ..



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    Figure 2: Co-immunoprecipitation experiments in HEK293 cells transfected with (A) pEF-BOS-SNAP25-FLAG alone or together with <t>pEGFPC1-TrkB.</t> (B) pEF-BOS-VAMP2-FLAG alone or together with pEGFPC1-TrkB. (C) pEF-BOS-SNAP25-FLAG, pEF-BOS- VAMP2-FLAG and TrkA-HA. (D) pEF-BOS-SNAP25-FLAG, pEF-BOS-VAMP2-FLAG and TrkC-myc. No co-immunoprecipitation was observed between proteins analyzed with anti α-GFP, anti-myc, anti-FLAG, or anti-HA antibodies. Two transfections in HEK cells per condition were run in parallel for each experiment and three experiments were done. Arrows indicate specific bands.
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    Image Search Results


    Figure 2: Co-immunoprecipitation experiments in HEK293 cells transfected with (A) pEF-BOS-SNAP25-FLAG alone or together with pEGFPC1-TrkB. (B) pEF-BOS-VAMP2-FLAG alone or together with pEGFPC1-TrkB. (C) pEF-BOS-SNAP25-FLAG, pEF-BOS- VAMP2-FLAG and TrkA-HA. (D) pEF-BOS-SNAP25-FLAG, pEF-BOS-VAMP2-FLAG and TrkC-myc. No co-immunoprecipitation was observed between proteins analyzed with anti α-GFP, anti-myc, anti-FLAG, or anti-HA antibodies. Two transfections in HEK cells per condition were run in parallel for each experiment and three experiments were done. Arrows indicate specific bands.

    Journal: Oncotarget

    Article Title: Syntaxin-1/TI-VAMP SNAREs interact with Trk receptors and are required for neurotrophin-dependent outgrowth.

    doi: 10.18632/oncotarget.26307

    Figure Lengend Snippet: Figure 2: Co-immunoprecipitation experiments in HEK293 cells transfected with (A) pEF-BOS-SNAP25-FLAG alone or together with pEGFPC1-TrkB. (B) pEF-BOS-VAMP2-FLAG alone or together with pEGFPC1-TrkB. (C) pEF-BOS-SNAP25-FLAG, pEF-BOS- VAMP2-FLAG and TrkA-HA. (D) pEF-BOS-SNAP25-FLAG, pEF-BOS-VAMP2-FLAG and TrkC-myc. No co-immunoprecipitation was observed between proteins analyzed with anti α-GFP, anti-myc, anti-FLAG, or anti-HA antibodies. Two transfections in HEK cells per condition were run in parallel for each experiment and three experiments were done. Arrows indicate specific bands.

    Article Snippet: Full-length Sytx1A or truncated DNAs (Sytx1 ACYT, Sytx1AHabc, Sytx1AH3TM, Sytx1AH3, and Sytx1ATM) were cloned into EcoRI sites in pEGFPC1 fusion mammalian protein expression vector (Clontech), as described [18].

    Techniques: Immunoprecipitation, Transfection

    Journal: eLife

    Article Title: SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA

    doi: 10.7554/eLife.74940

    Figure Lengend Snippet:

    Article Snippet: Recombinant DNA reagent , pEGFPC1-6XHis- FLKSRP (plasmid) , , RRID: Addgene_23001 , pEGFPC1 expression vector.

    Techniques: Protease Inhibitor, Recombinant, Clone Assay, Bicinchoninic Acid Protein Assay, Mutagenesis, Transfection, Sequencing, esiRNA, Blocking Assay, Plasmid Preparation, Expressing, Software